Coding

Part:BBa_K855005:Design

Designed by: Ma Tsz Shan   Group: iGEM12_HKU_HongKong   (2012-10-02)


pvdQ gene with a silent mutation at 1491 bp to remove the internal PstI site


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1196
    Illegal XhoI site found at 1594
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 31
    Illegal NgoMIV site found at 770
    Illegal NgoMIV site found at 1172
    Illegal NgoMIV site found at 1329
    Illegal NgoMIV site found at 1556
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1926


Design Notes

A his tag is added at the C-terminal of the gene sequence to help the detection of the expressed protein.


Source

pseudomonas aeruginosa PAO1

References